编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

U9 Broth (Urease Color Test Medium)

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

U9 Broth

(Urease Color Test Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 101.6mL:

U9 base ....................................................................................95.0mL

Horse serum, unheated...............................................................5.0mL

Penicillin G solution ..................................................................1.0mL

Urea solution..............................................................................0.5mL

Phenol Red solution ...................................................................0.1mL

pH 6.0 ± 0.2 at 25°C

U9 Base:

Composition per 100.0mL:

NaCl ............................................................................................ 0.63g

Pancreatic digest of casein........................................................ 0.425g

Papaic digest of soybean meal .................................................. 0.075g

K2HPO4..................................................................................... 0.063g

Glucose ..................................................................................... 0.063g

KH2PO4....................................................................................... 0.02g

Preparation of U9 Base: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5

with 1N HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Penicillin G Solution:

Composition per 10.0mL:

Penicillin G ................................................................................. 0.63g

Preparation of Penicillin G Solution: Add penicillin G to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Urea Solution:

Composition per 30.0mL:

Urea............................................................................................... 3.0g

Preparation of Urea Solution: Add urea to distilled/deionized

water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Phenol Red Solution:

Composition per 10.0mL:

Phenol Red.................................................................................... 0.1g

Preparation of Phenol Red Solution: Add Phenol Red to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: To 95.0mL of cooled, sterile U9 base,

aseptically add 5.0mL of sterile horse serum, 1.0mL of sterile penicil

lin G solution, 0.5mL of sterile urea solution, and 0.1mL of sterile Phe

nol Red solution. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the isolation and identification of T-strain mycoplasmas from

clinical specimens, especially Ureaplasma urealyticum. T-mycoplas

mas are the only members of the Mycoplasma group known to contain

urease. Bacteria with urease activity turn the medium dark pink.

在线客服

U9 Broth (Urease Color Test Medium)