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U9B Broth

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]
U9B Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 102.1mL:

U9 base ....................................................................................95.0mL
Horse serum, unheated...............................................................5.0mL
Penicillin G solution ..................................................................1.0mL
Urea solution..............................................................................0.5mL
L-Cysteine·HCl·H2O solution ....................................................0.5mL
Phenol Red solution...................................................................0.1mL
pH 6.0 ± 0.2 at 25°C
U9 Base:
Composition per 100.0mL:
NaCl ............................................................................................ 0.63g
Pancreatic digest of casein........................................................ 0.425g
Papaic digest of soybean meal .................................................. 0.075g
K2HPO4..................................................................................... 0.063g
Glucose ..................................................................................... 0.063g
KH2PO4....................................................................................... 0.02g
Preparation of U9 Base: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5
with 1N HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Penicillin G Solution:
Composition per 10.0mL:
Penicillin G ................................................................................ 0.63 g
Preparation of Penicillin G Solution: Add penicillin G to dis
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Urea Solution:
Composition per 30.0mL:
Urea............................................................................................... 3.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa
ter and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.
L-Cysteine·HCl·H2O Solution:
Composition per 50.0mL:
L-Cysteine·HCl·H2O ..................................................................... 1.0g
Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste
ine·HCl·H2O to distilled/deionized water and bring volume to 50.0mL.
Mix thoroughly. Filter sterilize.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red.................................................................................... 0.1g
Preparation of Phenol Red Solution: Add Phenol Red to dis
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: To 95.0mL of cooled, sterile U9 base,
aseptically add 5.0mL of sterile horse serum, 1.0mL of sterile penicil
lin G solution, 0.5mL of sterile urea solution, 0.5mL of sterile L
cysteine·HCl·H2O solution, and 0.1mL of sterile Phenol Red solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and identification of T-strain mycoplasmas from
clinical specimens, especially Ureaplasma urealyticum. T-mycoplas
mas are the only members of the Mycoplasma group known to contain
urease. Bacteria with urease activity turn the medium dark pink.
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