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Urea Agar

[所属分类:培养基配方] [发布时间:2021-9-30] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Urea............................................................................................. 10.0g

Na2HPO4·12H2O .......................................................................... 9.0g

NaCl.............................................................................................. 5.0g

KH2PO4 ........................................................................................ 1.5g

Meat extract .................................................................................. 1.0g

Yeast extract.................................................................................. 1.0g

MgSO4·7H2O................................................................................ 0.2g

MnCl2·4H2O ............................................................................ 20.0mg

CaCl2 ......................................................................................... 1.2mg

Glucose solution ....................................................................100.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize. Warm to 50°C.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of

sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the cultivation and maintenance of Corynebacterium glutam

icum.