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Urea Nutrient Agar

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Nutrient Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Agar ............................................................................................ 15.0g

Peptone ......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 2.0g

Beef extract................................................................................... 1.0g

Urea solution............................................................................50.0mL

Urea Solution:

Composition per 100.0mL:

Urea............................................................................................. 20.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile urea

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of Bacillus pantothenticus.