称取本品1.443g于1L蒸馏水或去离子水中，补充1支SD038和2支SD039，搅拌溶解，分装，121℃高压灭菌15分钟，冷至25℃，使用无菌10%碳酸氢钠溶液调节pH至7.8，备用。

Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile
10% NaHCO3 (medium is turning from yellow to pink).
Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes
to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES
may be added to keep pH neutral for a longer time.
Aeration must be adjusted according to the density and activity of the culture. At low cell
concentrations (which is the case most of the time) cells are sensitive to strong aeration
(don’t shake the cultures).