- 定做培养基/定制培养基
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DSMZ培养基1583,氨氧化细菌培养基,DSMZ 1583
DSMZ Medium 1583/DSMZ Medium1583/DSMZ 1583/DSMZ1583
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配套试剂
- 1. SD038 - 微量元素补充剂(DSMZ 1583) Trace element solution for DSMZ Medium 1583/DSMZ Medium 1583/DSMZ Medium1583/DSMZ 1583/DSMZ1583
- 2. SD039 - 0.05%甲酚红溶液 Cresol red solution
产品详情
培养基配方(每升)
NH4Cl 535mg
KH2PO4 54mg
KCl 74mg
MgSO4·7H2O 49mg
CaCl2·2H2O 147mg
NaCl 584mg
KH2PO4 54mg
KCl 74mg
MgSO4·7H2O 49mg
CaCl2·2H2O 147mg
NaCl 584mg
使用说明
称取本品1.443g于1L蒸馏水或去离子水中,补充1支SD038和2支SD039,搅拌溶解,分装,121℃高压灭菌15分钟,冷至25℃,使用无菌10%碳酸氢钠溶液调节pH至7.8,备用。
Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile
10% NaHCO3 (medium is turning from yellow to pink).
Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes
to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES
may be added to keep pH neutral for a longer time.
Aeration must be adjusted according to the density and activity of the culture. At low cell
concentrations (which is the case most of the time) cells are sensitive to strong aeration
(don’t shake the cultures).
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