- 定做培养基/定制培养基
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Leptospirillum培养基/HH培养基,DSMZ培养基882
Leptospirillum(HH)Medium,Leptospirillum Medium/HH Medium,DSMZ medium 882/DSMZ 882
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产品详情
产品详情
882: LEPTOSPIRILLUM (HH) MEDIUM
1. Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by
autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements
solution. The final pH of the medium should be 1.8.
2. Note: At the beginning of growth incubate statically without shaking.
For DSM 27195: Adjust pH of complete medium to 2.0.
Solution A
Solution B
Solution C
Trace element solution 1.00 ml
Trace element solution
|
Solution A Solution B Solution C |
950.00 50.00 1.00 |
ml ml ml |
autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements
solution. The final pH of the medium should be 1.8.
2. Note: At the beginning of growth incubate statically without shaking.
For DSM 27195: Adjust pH of complete medium to 2.0.
Solution A
|
(NH4)2SO4 MgCl2 x 6 H2O KH2PO4 CaCl2 x 2 H2O Distilled water Adjust pH to 1.8 with 10 N H2SO4. |
132.00 53.00 27.00 147.00 950.00 |
mg mg mg mg ml |
|
FeSO4 x 7 H2O H2SO4 (0.25 N) The pH of this solution should be 1.2. |
20.00 50.00 |
g ml |
Trace element solution 1.00 ml
Trace element solution
|
MnCl2 x 4 H2O ZnCl2 CoCl2 x 6 H2O H3BO3 Na2MoO4 CuCl2 x 2 H2O Distilled water |
76.00 68.00 64.00 31.00 10.00 67.00 1000.00 |
mg mg mg mg mg mg ml |
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