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DSMZ培养基383组分A基础/DSMZ383组分A基础
Desulfobacterium Medium Base,DSMZ medium 383 Solution A Base,DSMZ Medium 383/DSMZ Medium383/DSMZ 383/DSMZ383
产品编号: MD409
产品名称: DSMZ培养基383组分A基础/DSMZ383组分A基础
英文名称: Desulfobacterium Medium Base
DSMZ medium 383 Solution A Base
DSMZ Medium 383/DSMZ Medium383/DSMZ 383/DSMZ383
生产厂家: 山东拓普生物工程有限公司
产品用途: 用于制备DSMZ培养基383
浏览次数: 306
 
编号 规格 贮藏条件 保存期限 目录价 会员价 数量
MD409B250g/瓶常温3年请登录请登录请登录
配套试剂
产品详情
培养基配方(每升)
Na2SO4                    3.0g   
KH2PO4                       0.2g       
NH4Cl                        0.3g
NaCl                        21.0g
MgCl2·6H2O                  3.0g
KCl                          0.5g
CaCl2·2H2O                 0.15g
Sodium resazurin(刃天青钠)0.0005g    
使用说明
称取本品28.15g于956ml去离子水中,加入微量元素溶液SL-10(SD029)和亚硒酸盐-钨酸盐溶液(SD052)各1支,搅拌溶解,通入80%H2和20%CO2的混合气体不低于30分钟使pH低于6.0,装入厌氧容器中,121℃灭菌15分钟,冷却,备用。
制备DSMZ培养基383时,另制备并加入溶液B(1g碳酸钠、20ml水,121℃灭菌15分钟)、溶液C(2.5g丙酮酸钠、20ml水,过滤除菌)、溶液D(1支SD092)以及溶液E(取4ml SD104),混合,使用无菌的酸碱溶液调节pH至7.0-7.2,备用

特殊说明
383: DESULFOBACTERIUM MEDIUM
Solution A
Solution B
Solution C
Solution D
Solution E
952.00
20.00
20.00
1.00
10.00
ml
ml
ml
ml
ml
1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at
least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same
gas atmosphere. Solution B is autoclaved separately under 80% N
2 and 20% CO2 gas
atmosphere. Solutions C and D are prepared under 100% N
2 gas and filter-sterilized.
Solution E is autoclaved under 100% N
2 gas. To complete the medium appropriate
amounts of solutions B to E are added to the sterile solution A in the sequence as
indicated. Final pH of the medium should be 7.0 – 7.2.
2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N
2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 2056: Replace pyruvate with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate, and 0.15
g/l sodium octanoate.
For DSM 3383: Replace pyruvate with indole as substrate. Dissolve 0.30 g of indole in 90
ml water by heating and shaking in a closed bottle under 100% N
2 gas atmosphere,
autoclave, then add 7.00 ml of a sterile anoxic stock solution of NaCl (30% w/v) and 0.70
ml of a sterile anoxic stock solution of MgCl
2 x 6 H2O (40% w/v). Store the indole-salt
solution in the dark. Reheat and shake before use. Add to sterile medium 30.00 ml/l of the
indole-salt solution in the beginning, and 2 x 30.00 ml/l during growth.
For DSM 3384: The vitamin solution of medium 120 is replaced with 1.00 ml/l of seven
vitamins solution of medium 503. Replace pyruvate with 0.40 g/l Na-benzoate and add
10.00 ml/l clarified rumen fluid (see medium 1310) as growth supplement. Initiation of
growth requires addition of freshly prepared sodium dithionite.
For DSM 4661: Replace pyurvate with resorcinol. During growth the culture is fed once
with the same amount of resorcinol.
For DSM 5091, DSM 9788: Replace pyruvate with 2.00 g/l malonic acid.
For DSM 7044, DSM 7120, DSM 7467, DSM 12567, DSM 13228, DSM 23484: Supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503). Replace pyruvate with
0.40 g/l benzoate and 0.10 g/l yeast extract sterilized separately by filtration and add to
the autoclaved medium from anoxic stock solutions.
For DSM 8540: Replace pyruvate with 0.30 g/l p-hydroxybenzoate.
For DSM 9705: Replace pyruvate with 1.00 g/l Na-glycolate sterilized by filtration.

For DSM 12861, DSM 12883: Replace pyruvate with 1.00 g/l valeric acid.
For DSM 12888: Replace pyruvate with 1.10 g/l Na-butyrate and 1.10 g/l caproic acid.
For DSM 13036: Replace pyruvate with 1.00 g/l betaine.
For DSM 14454: Naphthalene dissolved in heptamethylnonane can be used as substrate
instead of pyruvate.
For DSM 15576, DSM 16219: Replace pyruvate with 1.00 g/l Na-caprylate and supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved
medium from an anoxic stock solution sterilized by filtration.
For DSM 17291: Replace pyruvate with 3.00 g/l Casamino acids.
For DSM 17477: Replace pyruvate with 1.90 g/l sodium glutamate monohydrate and 1.00
g/l yeast extract.
For DSM 21856: Replace pyruvate with 0.90 ml/l 1-butanol.
For DSM 28890: Replace pyruvate with 0.50 g/l yeast extract and 0.68 g/l sodium formate.
For DSM 100305, DSM 105015: Change amount of pyruvate to 2.20 g/l and add 0.10 g/l
yeast extract.
Solution A
Na2SO4
KH2PO4
NH4Cl
NaCl
MgCl
2 x 6 H2O
KCl
CaCl
2 x 2 H2O
Trace element solution SL-10
Selenite-tungstate solution
Sodium resazurin (0.1% w/v)
Distilled water
3.00
0.20
0.30
21.00
3.00
0.50
0.15
1.00
1.00
0.50
950.00
g
g
g
g
g
g
g
ml
ml
ml
ml
Solution B
Na2CO3
Distilled water
1.00
20.00
g
ml
Solution C
Na-pyruvate
Distilled water
2.50
20.00
g
ml
Solution D
Wolin's vitamin solution (10x) 1.00 ml
Solution E
Na2S x 9 H2O
Distilled water
0.40
10.00
g
ml
Selenite-tungstate solution (from medium 385)
NaOH
Na
2SeO3 x 5 H2O
Na
2WO4 x 2 H2O
Distilled water
0.50
3.00
4.00
1000.00
g
mg
mg
ml
Trace element solution SL-10 (from medium 320)
HCl (25%)
FeCl
2 x 4 H2O
ZnCl
2
MnCl2 x 4 H2O
H
3BO3
CoCl2 x 6 H2O
CuCl
2 x 2 H2O
NiCl
2 x 6 H2O
Na
2MoO4 x 2 H2O
Distilled water
10.00
1.50
70.00
100.00
6.00
190.00
2.00
24.00
36.00
990.00
ml
g
mg
mg
mg
mg
mg
mg
mg
ml
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
Biotin
Folic acid
Pyridoxine hydrochloride
Thiamine HCl
Riboflavin
Nicotinic acid
Calcium D-(+)-pantothenate
Vitamin B
12
p-Aminobenzoic acid
(DL)-alpha-Lipoic acid
Distilled water
20.00
20.00
100.00
50.00
50.00
50.00
50.00
1.00
50.00
50.00
1000.00
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
ml

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