斯通改良洛克氏液/斯通改良洛克氏溶液,Stone's Modification of Locke's Solution-定做培养基/定制培养基 -山东拓普生物工程有限公司

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斯通改良洛克氏液/斯通改良洛克氏溶液

Stone's Modification of Locke's Solution

产品编号：	MD591
产品名称：	斯通改良洛克氏液/斯通改良洛克氏溶液
英文名称：	Stone's Modification of Locke's Solution
生产厂家：	山东拓普生物工程有限公司
产品用途：	用于制备ATCC培养基1671；ATCC Medium: 1671 Blastocystis Egg Medium
浏览次数：	53

编号	规格	贮藏条件	保存期限	目录价	会员价	数量
MD591B	250g/瓶	常温	3年	请登录	请登录	请登录

产品详情

培养基配方（每升）

NaCl 8.0g
CaCl2 0.2g
KCl 0.2g
MgCl2·6H2O 0.01g
Na2HPO4 2.0g
NaHCO3 0.4g
KH2PO4 0.3g

使用说明

称取本品11.11g于1L蒸馏水中，121℃高压灭菌15分钟或115℃灭菌30分钟，冷却，按文件规定的方法制备ATCC培养基1671.
本品有不溶物，分装时需搅拌使用。

特殊说明

ATCC Medium: 1671 Blastocystis Egg Medium

Whole emulsified egg Stone’s Modification of Locke’s Solution (see below)

783 217

mlml

Use only fresh fertile eggs (one source for such eggs is B&E eggs in York Spring Pa. Ship at Day 0)

Filter through gauze into a filtration flask.

Place under vacuum to draw out air bubbles.

Dispense in 4.0 ml amounts to a 16 x 125 mm screw-cap tube.

Inspissate (80 °C) at a 30 degree slant .Generally it takes approximately 1 hour to set. Remove when eggs have solidified. Be careful not to overcook.

Allow to cool and add a 4.5 ml overlay of Stone’s Modification of Locke’s Solution.

Cap with rubber stoppers then autoclave for 20 minutes at 121°C.

Remove rubber stoppers after the medium is cooled the aseptically replace with screw-caps.

Stone’s Modification of Locke’s Solution*:

NaCl CaCl2 KCl MgCl2 x 6H2O Na2HPO4 NaHCO3 KH2PO4 Distilled water	8.0 0.2 0.2 0.01 2.0 0.4 0.3	ggggggg
1000.0 ml

*Reference: Am. J. Trop. Med., 15, 681- , 1935.

Note: Solution will precipitate when Na2HPO4 is added. Dispense while stirring to evenly distribute salts.

Protistology will add 25% (v/v) heat-inactivated (56C for 30 minutes) horse serum (~1.5ml) to each tube just before use (before placing the tubes in the anaerobic jar 48 hours prior to inoculation with Blastocystis).

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