- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
定制CPC琼脂基础组分A,CPC培养基
CPC medium
  |
|
配套试剂
- 1. S0203 - 25%纤维二糖溶液（0.5g/支） Cellobiose solution
- 2. SD220 - 多粘菌素B溶液 Polymyxin B solution
- 3. SD221 - 甲磺酸黏菌素溶液 colistin methanesulfonate solution
产品详情
培养基配方（每升）
Bacto peptone（蛋白胨） 10.0g
proteose peptone（䏡蛋白胨） 5.0g
NaCl 10.0g
MgCl2·6H2O 4.0g
KCl 4.0g
Bromothymol blue
（溴麝香草酚蓝） 0.04g
Cresol red（甲酚红） 0.04g
Agar（琼脂） 15.0g
pH7.6±0.2（25℃）
proteose peptone（䏡蛋白胨） 5.0g
NaCl 10.0g
MgCl2·6H2O 4.0g
KCl 4.0g
Bromothymol blue
（溴麝香草酚蓝） 0.04g
Cresol red（甲酚红） 0.04g
Agar（琼脂） 15.0g
pH7.6±0.2（25℃）
使用说明
称取本品48.0g于900ml蒸馏水中，加热煮沸至完全溶解，90ml/瓶分装，保温至55-60℃。无菌操作，每90ml培养基中添加3支S0203、1支SD220和1支SD221，轻轻混匀，倾注平皿，凝固，备用。
特殊说明
CPC medium is prepared from two solutions. Solution A
contains Bacto peptone (10 g), proteose peptone (5 g), NaCl
(10 g), MgCl2 6H2O (4 g), KCl (4 g), bromothymol blue (0.04
g), cresol red (0.04 g), and distilled water (900 ml). The pH is
adjusted to 7.6, and 15 g of agar is added. The solution is
autoclaved for 20 min and then cooled to between 55 and 60°C.
Solution B contains D-cellobiose (15 g), colistin methanesul-
fonate (1 106 units), and polymyxin B (4 105 units) dis-
solved in 100 ml distilled water by heat treatment at a low
temperature. This solution is cooled to 55 to 60°C before the
antibiotics are added and then filter sterilized and added to
solution A.
contains Bacto peptone (10 g), proteose peptone (5 g), NaCl
(10 g), MgCl2 6H2O (4 g), KCl (4 g), bromothymol blue (0.04
g), cresol red (0.04 g), and distilled water (900 ml). The pH is
adjusted to 7.6, and 15 g of agar is added. The solution is
autoclaved for 20 min and then cooled to between 55 and 60°C.
Solution B contains D-cellobiose (15 g), colistin methanesul-
fonate (1 106 units), and polymyxin B (4 105 units) dis-
solved in 100 ml distilled water by heat treatment at a low
temperature. This solution is cooled to 55 to 60°C before the
antibiotics are added and then filter sterilized and added to
solution A.
相关产品
- 1. MD427 - 定做培养基
- 2. SD168 - ATCC MD-VS维生素补充溶液/ ATCC MD-VS维生素溶液/ATCC MDVS维生素补充溶液 ATCC MD-VS Vitamin Supplement
- 3. MD523 - 定做培养基
- 4. MD163 - 乳酸菌培养基I Lactic Bacteria Medium I
- 5. MD041 - 营养琼脂(pH6.0) Nutrient Agar
- 6. MD072 - 甘露醇氯化钠液体培养基 Mannitol Salt Broth MediumMSB
- 7. SD139 - 10%葡萄糖溶液（0.5g/支）
- 8. MD012 - 0787 强化梭菌培养基/强化梭菌琼脂培养基 Reinforced Clostridium Medium（RCM）
- 9. SD113 - DSMZ培养基204补充剂
- 10. MD178 - PY+X培养基（含琥珀酸钠） PY+X Medium with Na2-succinateDSMZ Medium 104c
