- 定做培养基/定制培养基
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定制CPC琼脂基础组分A,CPC培养基
CPC medium
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配套试剂
- 1. S0203 - 25%纤维二糖溶液（0.5g/支） Cellobiose solution
- 2. SD220 - 多粘菌素B溶液 Polymyxin B solution
- 3. SD221 - 甲磺酸黏菌素溶液 colistin methanesulfonate solution
产品详情
培养基配方（每升）
Bacto peptone（蛋白胨） 10.0g
proteose peptone（䏡蛋白胨） 5.0g
NaCl 10.0g
MgCl2·6H2O 4.0g
KCl 4.0g
Bromothymol blue
（溴麝香草酚蓝） 0.04g
Cresol red（甲酚红） 0.04g
Agar（琼脂） 15.0g
pH7.6±0.2（25℃）
proteose peptone（䏡蛋白胨） 5.0g
NaCl 10.0g
MgCl2·6H2O 4.0g
KCl 4.0g
Bromothymol blue
（溴麝香草酚蓝） 0.04g
Cresol red（甲酚红） 0.04g
Agar（琼脂） 15.0g
pH7.6±0.2（25℃）
使用说明
称取本品48.0g于900ml蒸馏水中，加热煮沸至完全溶解，90ml/瓶分装，保温至55-60℃。无菌操作，每90ml培养基中添加3支S0203、1支SD220和1支SD221，轻轻混匀，倾注平皿，凝固，备用。
特殊说明
CPC medium is prepared from two solutions. Solution A
contains Bacto peptone (10 g), proteose peptone (5 g), NaCl
(10 g), MgCl2 6H2O (4 g), KCl (4 g), bromothymol blue (0.04
g), cresol red (0.04 g), and distilled water (900 ml). The pH is
adjusted to 7.6, and 15 g of agar is added. The solution is
autoclaved for 20 min and then cooled to between 55 and 60°C.
Solution B contains D-cellobiose (15 g), colistin methanesul-
fonate (1 106 units), and polymyxin B (4 105 units) dis-
solved in 100 ml distilled water by heat treatment at a low
temperature. This solution is cooled to 55 to 60°C before the
antibiotics are added and then filter sterilized and added to
solution A.
contains Bacto peptone (10 g), proteose peptone (5 g), NaCl
(10 g), MgCl2 6H2O (4 g), KCl (4 g), bromothymol blue (0.04
g), cresol red (0.04 g), and distilled water (900 ml). The pH is
adjusted to 7.6, and 15 g of agar is added. The solution is
autoclaved for 20 min and then cooled to between 55 and 60°C.
Solution B contains D-cellobiose (15 g), colistin methanesul-
fonate (1 106 units), and polymyxin B (4 105 units) dis-
solved in 100 ml distilled water by heat treatment at a low
temperature. This solution is cooled to 55 to 60°C before the
antibiotics are added and then filter sterilized and added to
solution A.
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