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YA Halophile Medium

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

YA Halophile Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl.......................................................................................... 100.0g

Agar ............................................................................................ 15.0g

Sodium acetate·3H2O.................................................................. 10.0g

Na2HPO4....................................................................................... 3.8g

KH2PO4......................................................................................... 1.3g

Mg(NO3)2·6H2O ........................................................................... 1.0g

(NH4)2SO4 .................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except magnesium ni

trate, to tap water and bring volume to 1.0L. Mix thoroughly. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically add magnesium nitrate. Adjust pH 7.2 with sterile KOH.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of halophilic microorgan

isms, including Bacillus halodenitrificans.