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YB Medium (Yeast Extract Beef Extract Medium)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

YB Medium

(Yeast Extract Beef Extract Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Peptone ....................................................................................... 10.0g

Beef extract................................................................................... 7.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 3.0g

Thiourea........................................................................................ 0.1g

Methanol..................................................................................20.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except methanol, to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add filter-sterilized methanol. Pour into sterile Petri

dishes or leave in tubes.

Use: For the cultivation and maintenance of bacteria that can utilize

methanol as a carbon source, including Achromobacter methanolo

phila, Methanomonas methylovora, Methylobacterium species, and

Pseudomonas methanolica.