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YC Agar without Tryptophan

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................500.0mL

Solution B ..............................................................................500.0mL

Solution A:

Composition per 500.0mL:

Glucose ....................................................................................... 20.0g

Succinic acid ............................................................................... 10.0g

NaOH ............................................................................................ 6.0g

(NH4)2SO4 .................................................................................... 5.0g

KH2PO4......................................................................................... 0.6g

MgSO4·7H2O ................................................................................ 0.3g

Adenine......................................................................................... 0.1g

L-Arginine ..................................................................................... 0.1g

L-Cysteine ..................................................................................... 0.1g

L-Leucine ...................................................................................... 0.1g

L-Lysine......................................................................................... 0.1g

L-Threonine................................................................................... 0.1g

Uracil ............................................................................................ 0.1g

L-Aspartic acid............................................................................ 0.05g

L-Histidine................................................................................... 0.05g

L-Isoleucine................................................................................. 0.05g

L-Methionine............................................................................... 0.05g

L-Phenylalanine........................................................................... 0.05g

L-Proline...................................................................................... 0.05g

L-Serine....................................................................................... 0.05g

L-Tyrosine ................................................................................... 0.05g

L-Valine....................................................................................... 0.05g

NaCl ............................................................................................ 0.05g

CaCl2·2H2O................................................................................. 0.05g

DL-Methionine............................................................................. 0.01g

L-Histidine·HCl ......................................................................... 0.005g

Inositol .......................................................................................1.0mg

KI ...............................................................................................0.5mg

H3BO3 ........................................................................................0.3mg

ZnSO4·7H2O ..............................................................................0.2mg

MnSO4·4H2O .............................................................................0.2mg

Thiamine·HCl ............................................................................0.2mg

Pyroxidine·HCl ..........................................................................0.2mg

Niacin.........................................................................................0.2mg

Calcium pantothenate ................................................................0.2mg

p-Aminobenzoic acid.................................................................0.1mg

Riboflavin ..................................................................................0.1mg

FeCl3 ..........................................................................................0.1mg

Na2MoO4·4H2O .........................................................................0.1mg

CuSO4·5H2O ............................................................................0.02mg

Folic acid.....................................................................................1.0μg

Biotin ..........................................................................................1.0μg

pH 5.7 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to

5.6–5.7. Filter sterilize. Warm to 50°–55°C.

Solution B:

Composition per 500.0mL:

Agar, noble.................................................................................. 20.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Preparation of Medium: Aseptically mix 500.0mL of solution A

and 500.0mL of solution B. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevi

siae.

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YC Agar without Tryptophan