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培养基配方

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YC Medium without Tryptophan

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL

Solution B ..............................................................................500.0mL

Solution A:

Composition per 500.0mL:

Glucose ....................................................................................... 20.0g

Succinic acid............................................................................... 10.0g

NaOH............................................................................................ 6.0g

(NH4)2SO4 .................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

Adenine......................................................................................... 0.1g

L-Arginine..................................................................................... 0.1g

L-Cysteine ..................................................................................... 0.1g

L-Leucine ...................................................................................... 0.1g

L-Lysine ........................................................................................ 0.1g

L-Threonine................................................................................... 0.1g

Uracil ............................................................................................ 0.1g

L-Aspartic acid............................................................................ 0.05g

L-Histidine .................................................................................. 0.05g

L-Isoleucine................................................................................. 0.05g

L-Methionine............................................................................... 0.05g

L-Phenylalanine .......................................................................... 0.05g

L-Proline ..................................................................................... 0.05g

L-Serine....................................................................................... 0.05g

L-Tyrosine ................................................................................... 0.05g

L-Valine....................................................................................... 0.05g

Inositol ....................................................................................... 2.0mg

KI ............................................................................................... 1.0mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyroxidine·HCl .......................................................................... 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

FeCl3 .......................................................................................... 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Adjust pH to 5.6–5.7. Filter ster

ilize. Warm to 50°–55°C.

Solution B:

Composition per 500.0mL:

Agar, noble.................................................................................. 20.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 500.0mL. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50–55°C.

Preparation of Medium: Aseptically combine 500.0mL of solu

tion A and 500.0mL of solution B. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of Saccharomyces cerevisiae.

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YC Medium without Tryptophan