培养基配方

您现在的位置：网站首页 >> 培养基配方

Yeast Extract Glucose Agar

[所属分类:培养基配方] [发布时间:2021-10-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Glucose ....................................................................................... 15.0g

K2HPO4 ........................................................................................ 5.2g

KH2PO4 ...................................................................................... 3.18g

NH4Cl ......................................................................................... 0.54g

Yeast extract.................................................................................. 0.5g

MgSO4 ........................................................................................ 0.12g

Trace elements solution .............................................................5.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

ZnSO4·7H2O............................................................................. 0.287g

CuSO4·5H2O............................................................................. 0.249g

MnSO4·4H2O............................................................................ 0.223g

Na2MoO4·2H2O........................................................................ 0.124g

CaCl2·6H2O .............................................................................. 0.118g

KJ.............................................................................................. 0.083g

H3BO3......................................................................................... 0.03g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of Bacillus licheniformis, Bacil

lus species, Clavibacter michiganense, Flavobacterium indologenes, Haf

nia alvei, Pseudomonas fluorescens, and Serratia marcescens.