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Yeast Extract Glucose Broth

[所属分类:培养基配方] [发布时间:2021-10-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 15.0g

K2HPO4 ........................................................................................ 5.2g

KH2PO4 ...................................................................................... 3.18g

NH4Cl ......................................................................................... 0.54g

Yeast extract.................................................................................. 0.5g

MgSO4 ........................................................................................ 0.12g

Trace elements solution .............................................................5.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

ZnSO4·7H2O............................................................................. 0.287g

CuSO4·5H2O............................................................................. 0.249g

MnSO4·4H2O............................................................................ 0.223g

Na2MoO4·2H2O........................................................................ 0.124g

CaCl2·6H2O .............................................................................. 0.118g

KJ.............................................................................................. 0.083g

H3BO3......................................................................................... 0.03g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and maintenance of Bacillus licheniformis, Bacil

lus species, Clavibacter michiganense, Flavobacterium indologenes, Haf

nia alvei, Pseudomonas fluorescens, and Serratia marcescens.