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Yeast Nitrogen Base, 10X with Asparagine and Glucose

[所属分类:培养基配方] [发布时间:2021-10-20] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Nitrogen Base, 10X

with Asparagine and Glucose

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 10.0g

(NH4)2SO4 .................................................................................... 5.0g

L-Asparagine ................................................................................. 1.5g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol ....................................................................................... 2.0mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyridoxine·HCl .......................................................................... 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

FeCl3 .......................................................................................... 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

KI ............................................................................................... 0.1mg

CuSO4·5H2O............................................................................ 0.04mg

Folic acid ....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Dilute 100.0mL of 10X medium with

900.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Aseptically distribute into sterile tubes or flasks.

Use: For susceptibility tests with yeasts and fungi.