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YI-S Medium

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

YI-S Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

YI broth..................................................................................880.0mL

Bovine serum, heat inactivated..............................................100.0mL

Vitamin mixture 18................................................................. 20.0mL

Source: Vitamin mixture 18 is available from Bio-fluids, Inc., Rock

ville, MD.

YI Broth:

Composition per liter:

YI base stock......................................................................... 780.0mL

10X Glucose buffer stock ......................................................100.0mL

YI Base Stock:

Composition per 780.0mL:

Yeast extract................................................................................ 30.0g

L-Cysteine·HCl.............................................................................. 1.0g

NaCl .............................................................................................. 1.0g

Ascorbic acid ................................................................................ 0.2g

Ferric ammonium citrate........................................................228.0mg

10X Glucose Buffer Stock:

Composition per 100.0.0mL:

Glucose ....................................................................................... 10.0g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 0.6g

Preparation of 10X Glucose Buffer Stock: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Preparation of YI Base Stock: Add components to 600.0mL of

distilled/deionized water. Mix thoroughly. Bring volume to 780.0mL

with distilled/deionized water. Adjust pH to 6.8 with 1N NaOH. Dis

tribute in 78.0mL aliquots to 100.0mL screw-capped bottles. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Preparation of YI Broth: Aseptically add 10.0mL of 10X glucose

buffer stock to 78.0mL of cooled YI base stock. Adjust osmolarity with

NaCl to 380.0milliosmols/kg.

Preparation of Medium: Aseptically add 2.0mL of vitamin mix

ture 18 and 10.0mL of heat-inactivated bovine serum to 88.0mL of YI

broth. Distribute in 13.0mL aliquots to 16 x 125mm screw-capped test

tubes. Store at 4°C in the dark with the caps screwed on tightly. Use

within 96 hr.

Use: For the cultivation of Entamoeba species.