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YM-1L Broth

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

YM-1L Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium lactate ............................................................................ 30.0g

Mycological peptone .................................................................. 10.0g

Succinic acid............................................................................... 10.0g

NaOH............................................................................................ 6.0g

Yeast extract.................................................................................. 5.0g

Adenine....................................................................................... 0.01g

Uracil .......................................................................................... 0.01g

Yeast nitrogen base solution ..................................................100.0mL

Yeast Nitrogen Base Solution:

Composition per 100.0mL:

(NH4)2SO4 .................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol ....................................................................................... 2.0mg

KI ............................................................................................... 0.1mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyroxidine·HCl .......................................................................... 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

FeCl3 .......................................................................................... 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

CuSO4·5H2O............................................................................ 0.04mg

Folic acid ....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

Preparation of Yeast Nitrogen Base Solution: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except yeast nitrogen

base solution, to distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 1.0L of sterile yeast nitrogen base solution.

Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Saccharomyces cerevisiae.

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YM-1L Broth