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Yolk Milk Medium (YOM)

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

Yolk Milk Medium

(YOM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Egg yolk.................................................................................500.0mL

Milk........................................................................................500.0mL

Egg Yolk:

Composition per 500.0mL:

Chicken egg yolks................................................................... variable

Preparation of Egg Yolk: Soak eggs with 1:100 dilution of saturat

ed mercuric chloride solution for 1 min. Crack eggs and separate yolks

from whites. Add sufficient egg yolk to bring volume to 500.0mL. Mix

thoroughly.

Preparation of Milk: Autoclave 500.0mL of milk for 20 min at 15

psi pressure–115°C.

Preparation of Medium: Combine 500.0mL of sterile egg yolk and

500.0mL of sterile milk. Mix thoroughly. Distribute into sterile tubes

or flasks. Heat to 95°C for 20–25 min.

Use: For the cultivation of Condiobolus obscurus.