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Yopp’s Medium

[所属分类:培养基配方] [发布时间:2021-10-22] [发布人:网站管理员2] [阅读次数:] [返回]

Yopp’s Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl........................................................................................ 116.88g

MgCl2·6H2O ............................................................................. 10.68g

MgSO4·7H2O.............................................................................. 10.0g

KCl................................................................................................ 2.0g

CaNO3·4H2O ................................................................................ 1.0g

Glycyl-glycine buffer.................................................................... 0.5g

K2HPO4·3H2O .......................................................................... 0.065g

Ferric EDTA .............................................................................. 5.0mg

Trace metals solution .................................................................1.0mL

pH 7.8 ± 0.2 at 25°C

Trace Metals Solution:

Composition per liter:

MnCl2·4H2O ................................................................................. 2.0g

H3BO3 ........................................................................................... 0.5g

ZnNO3·6H2O ................................................................................ 0.5g

Co(NO3)2·6H2O ........................................................................ 0.025g

CuCl2·2H2O .............................................................................. 0.025g

Na2MoO4·2H2O ........................................................................ 0.025g

VOSO4·6H2O............................................................................ 0.025g

HCl.............................................................................................3.0mL

Preparation of Trace Metals Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For the isolation and cultivation of halophilic cyanobacteria.