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YTG Medium

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

YTG Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone...................................................................................... 10.0g

Na2CO3 ........................................................................................ 5.3g

Yeast extract.................................................................................. 5.0g

Na2HPO4·2H2O......................................................................... 0.356g

L-Cysteine·HCl ............................................................................. 0.2g

Na2S·9H2O.................................................................................... 0.2g

KCl............................................................................................ 0.075g

Resazurin ................................................................................... 1.0mg

Glucose solution ......................................................................20.0mL

pH 10.1 ± 0.2 at 25°C

Glucose Solution:

Composition per 20.0mL:

D-Glucose...................................................................................... 3.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 20.0mL. Mix thoroughly. Sparge with

100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except glucose solution, to distilled/deion

ized water and bring volume to 980.0L. Mix thoroughly. Sparge with

100% N2 for 30 min. Anaerobically distribute 9.8mL volumes into

tubes. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and

anaerobically add 0.2mL of sterile glucose solution to each tube. Ad

just pH to 10.1 with sterile anaerobic 3N NaOH solution.

Use: For the cultivation of Clostridium paradoxum and Clostridium

thermoalcaliphilum.