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YTN Medium (Yeast Extract Tryptone NaCl Medium)

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

YTN Medium

(Yeast Extract Tryptone NaCl Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 30.0g

Agar ............................................................................................ 15.0g

Yeast extract................................................................................ 10.0g

Pancreatic digest of casein.......................................................... 10.0g

Glucose ......................................................................................... 1.0g

Trace elements solution .............................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

H3BO3 ......................................................................................... 2.85g

MnCl2·4H2O.................................................................................. 1.8g

Sodium tartrate............................................................................ 1.77g

FeSO4 .......................................................................................... 1.36g

CoCl2·6H2O ................................................................................ 0.04g

CuCl2·2H2O............................................................................... 0.027g

Na2MoO4·2H2O ........................................................................ 0.025g

ZnCl2......................................................................................... 0.021g

pH 7.2 ± 0.2 at 25°C

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to tap water and bring

volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of ATCC strain 21588.