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Zymobacterium Agar

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

Zymobacterium Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Agar ............................................................................................ 15.0g

Orotic acid..................................................................................... 2.0g

Na3PO4·12H2O.............................................................................. 1.5g

Sodium thioglycolate .................................................................... 1.0g

NaOH ............................................................................................ 0.5g

Riboflavin ................................................................................. 0.015g

pH 7.9 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa

ter in the following order: tryptone, orotic acid, sodium hydroxide, sodium

phosphate, sodium thioglycolate, riboflavin, agar and bring volume to

1.0L . Mix thoroughly. Some orotic acid will remain undissolved. Adjust

pH to 7.9 using NaH2PO4.Distribute into tubes or flasks. Autoclave for 15

min at 15 psi pressure–121°C. Mix thoroughly to dissolve orotic acid. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Clostridium (Zymobacte

rium) oroticum.