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Zymobacterium Broth

[所属分类:培养基配方] [发布时间:2021-10-25] [发布人:网站管理员2] [阅读次数:] [返回]

Zymobacterium Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Orotic acid..................................................................................... 2.0g

Na3PO4·12H2O.............................................................................. 1.5g

Sodium thioglycolate .................................................................... 1.0g

NaOH ............................................................................................ 0.5g

Riboflavin ................................................................................. 0.015g

pH 7.9 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa

ter in the following order: tryptone, orotic acid, sodium hydroxide, sodium

phosphate, sodium thioglycolate, riboflavin and bring volume to 1.0L.

Mix thoroughly. Some orotic acid will remain undissolved. Adjust pH to

7.9 using NaH2PO4. Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Mix again to dissolve orotic acid.

Use: For the cultivation and maintenance of Clostridium (Zymobacte

rium) oroticum.