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Acetamide Medium (BAM M2)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetamide Medium

(BAM M2)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Stock basal solution .................................... 400.0mL

Stock acetamide solution ............................ 100.0mL

pH 6.9 ± 0.2 at 25°C

Stock Basal Solution:

Composition per 400.0mL:

Agar ....................................................................0.5g

KH2PO4 solution, 0.5M ................................ 14.0mL

K2HPO4 solution, 0.5M .................................. 6.0mL

Preparation of Stock Basal Solution: Add

components, except PR-CV solution, to distilled/

deionized water and bring volume to 400.0mL. Mix

thoroughly. Gently heat and bring to boiling with ag

itation to dissolve agar. Add 1.0mL PR-CV solution.

PR-CV Solution:

Composition per 200.0mL:

Phenol Red..........................................................2.0g

Crystal Violet......................................................0.2g

Preparation of PR-CV Solution: Add compo

nents to distilled/deionized water and bring volume

to 200.0mL. Mix thoroughly. Add 5N NaOH while

stirring until components are dissolved.

Stock Acetamide Solution:

Composition per 100.0mL:

Acetamide...........................................................1.0g

Preparation of Stock Acetamide Solution:

Add acetamide to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Store over me

thylene chloride in a screw capped container. Can be

stored indefinitely at room temperature.

Preparation of Medium: Combine 400.0mL

stock basal solution and 100.0mL stock acetamide

solution. Mix thoroughly. Distribute into tubes or

flasks. Steam for 10 min at 100°C. Cool.

Use: For the differentiation of nonfermentative

Gram-negative bacteria, especially Pseudomonas

aeruginosa, e.g., in milk.