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Acetate Agar (BAM M3)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetate Agar

(BAM M3)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

NaCl....................................................................5.0g

Sodium acetate....................................................2.0g

KH2PO4...............................................................1.0g

(NH4)2PO4 ..........................................................1.0g

MgSO4 ................................................................0.2g

Bromthymol blue..............................................0.08g

pH 6.7 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept MgSO4, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Adjust pH to 6.7. Add 0.2g MgSO4.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically distrib

ute into tubes or flasks. For slants distribute aliquots

into tubes prior to autoclaving. After autoclaving al

low tubes to cool in inclined position to obtain a 5cm

slant.

Use: For the cultivation of Leuconostoc species.