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Acetate Broth

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetate Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Meat extract ......................................................50.0g

Glucose .............................................................10.0g

Peptone................................................................5.0g

Yeast extract........................................................5.0g

Sodium acetate buffer ................................. 100.0mL

Tween 80......................................................... 0.5mL

pH 5.4 ± 0.2 at 25°C

Sodium Acetate Buffer:

Composition per liter:

Sodium acetate·3H2O......................................272.2g

Preparation of Sodium Acetate Buffer: Add

sodium acetate to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Adjust pH to 5.4

with glacial acetic acid. Filter sterilize.

Preparation of Medium: Add components, ex

cept sodium acetate buffer, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Adjust pH to 5.4.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 100.0mL of sterile so

dium acetate buffer. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Leuconos

toc species and Pediococcus species.