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Acetate Differential Agar (Sodium Acetate Agar) (Simmons’ Citrate Agar, Modified)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetate Differential Agar

(Sodium Acetate Agar)

(Simmons’ Citrate Agar, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

NaCl....................................................................5.0g

Sodium acetate....................................................2.0g

(NH4)H2PO4........................................................1.0g

K2HPO4...............................................................1.0g

MgSO4·7H2O ......................................................0.2g

Bromthymol Blue .............................................0.08g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

cold distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat and bring to boil

ing. Distribute into tubes to produce a 1 cm butt and

30 cm slant. Autoclave for 15 min at 15 psi pressure–

121°C. Cool tubes in a slanted position.

Use: For the differentiation of Shigella species from

Escherichia coli and also for the differentiation of

nonfermenting Gram-negative bacteria. Bacteria that

can utilize acetate as the sole carbon source turn the

medium blue.