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Acetobacter Agar

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Acetobacter Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Yeast extract........................................................5.0g

(NH4)2SO4 ..........................................................3.3g

KH2PO4 ..............................................................1.0g

MgSO4 ..............................................................0.25g

Vitamin solution.......................................... 200.0mL

Glucose solution ........................................... 15.0mL

Trace elements solution .................................. 1.0mL

Trace Elements Solution:

Composition per 100.0mL:

CaCl2·2H2O ....................................................1.457g

FeSO4·7H2O ...................................................0.366g

ZnSO4·7H2O...................................................0.178g

MnSO4·H2O....................................................0.101g

Na2MoO4·2H2O.............................................23.4mg

CuSO4·5H2O....................................................7.8mg

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Vitamin Solution:

Composition per 100.0mL:

m-Inositol.....................................................200.0mg

Calcium DL-pantothenate...............................40.0mg

Nicotinic acid.................................................40.0mg

Pyrdoxine·HCl ...............................................40.0mg

Thiamine·HCl ................................................40.0mg

p-Aminobenzoic acid.....................................20.0mg

Riboflavin ......................................................20.0mg

Biotin ...............................................................0.2mg

Folic acid .........................................................0.2mg

Preparation of Vitamin Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize.

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................40.0g

Preparation of Glucose Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept vitamin solution, glucose solution, and trace el

ement solution, to distilled/deionized water and bring

volume to 784.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Aseptically add

200.0mL of sterile vitamin solution, 15.0mL of ster

ile glucose solution, and 1.0mL of sterile trace el

ments solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Aceto

bacter xylinum.

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Acetobacter Agar