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Alcaligenes Agar
[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]
Alcaligenes Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................10.0g
Peptone................................................................5.0g
Ammonium lactate..............................................3.0g
Meat extract ........................................................3.0g
Ferric citrate........................................................0.2g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add ferric citrate to
distilled/deionized water and bring volume to
100.0mL. In a separate flask, add remaining compo
nents to distilled/deionized water and bring volume
to 900.0mL. Mix thoroughly. Adjust pH to 7.0.
Steam the two solutions for 20 min on three consec
utive days. Aseptically combine the two solutions.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of Alcaligenes species.