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Alcaligenes Medium

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Alcaligenes Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone................................................................5.0g

Beef extract.........................................................3.0g

Ferric citrate........................................................0.2g

Ammonium lactate solution............................ 3.0mL

pH 7.0 ± 0.2 at 25°C

Ammonium Lactate Solution:

Composition per 100.0mL:

Lactic acid.........................................................60.0g

Preparation of Ammonium Lactate Solu

tion: Dissolve lactic acid in 100.0mL of distilled/

deionized water. Neutralize with NH4OH to pH 7.0.

Preparation of Medium: Add peptone, beef ex

tract, and ammonium lactate to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Add ferric citrate

aseptically. Mix thoroughly. Aseptically distribute

into tubes or flasks.

Use: For the cultivation of Alcaligenes tolerans.