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Alkalophile Medium

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Alkalophile Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone ...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Beef extract.........................................................1.0g

Sodium sesquicarbonate solution ................. 15.0mL

pH 9.5 ± 0.2 at 25°C

Sodium Sesquicarbonate Solution:

Composition per 100.0mL:

Sodium sesquicarbonate .....................................9.0g

Preparation of Sodium Sesquicarbonate So

lution: Add sodium sesquicarbonate to distilled/

deionized water and bring volume to 100.0L. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept sodium sesquicarbonate solution, to distilled/

deionized water and bring volume to 985.0L. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 15.0mL of filter-steriled

sodium sesquicarbonate solution to adjust pH to 9.5.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of Bacillus alcalophilus,

Bacillus circulans, Bacillus submarinus, and other

Bacillus species.