培养基配方

您现在的位置：网站首页 >> 培养基配方

Alkvisco Medium

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Alkvisco Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Acrylonitrile........................................................0.5g

KCN...............................................................10.0mg

pH 6.5-8.0 at 25°C

Caution: Cyanide is toxic.

Preparation of Medium: Add components, ex

cept acrylonitrile, to distilled/deionized water and

bring volume to 980.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 10 min at 15

psi pressure–121°C. Add acrylonitrile to 20.0mL of

distilled/deionized water and filter sterilize. Add

aseptically to the sterile basal medium.

Caution: Acrylonitrile is a carcinogen; use appro

priate precautions.

Use: For the cultivation and maintenance of Bacillus

subtilis and Corynebacterium species.