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Amino Acid Assay Medium

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................50.0g

Sodium acetate..................................................40.0g

NH4Cl .................................................................6.0g

KH2PO4...............................................................1.2g

K2HPO4...............................................................1.2g

Asparagine ..........................................................0.8g

L-Glutamic acid...................................................0.6g

Pyridoxamine·HCl ..............................................0.6g

Pyridoxal·HCl .....................................................0.6g

DL-Valine .............................................................0.5g

L-Lysine·HCl .......................................................0.5g

DL-Isoleucine.......................................................0.5g

DL-Leucine ..........................................................0.5g

L-Arginine·HCl....................................................0.5g

DL-Threonine.......................................................0.4g

MgSO4·7H2O ......................................................0.4g

DL-Alanine...........................................................0.4g

DL-Phenylalanine.................................................0.2g

L-Tyrosine............................................................0.2g

Lysine..................................................................0.2g

L-Aspartic acid ....................................................0.2g

DL-Methionine.....................................................0.2g

L-Proline..............................................................0.2g

L-Histidine·HCl .................................................0.12g

DL-Serine .............................................................0.1g

L-Cystine .............................................................0.1g

DL-Tryptophan...................................................0.08g

MnSO4·7H2O ....................................................0.04g

FeSO4 ................................................................0.02g

NaCl..................................................................0.02g

Adenine sulfate .................................................0.02g

Guanine·HCl .....................................................0.02g

Uracil ................................................................0.02g

Xanthine............................................................0.02g

Nicotinic acid...................................................2.0mg

Pyridoxine·HCl ................................................2.0mg

Thiamine·HCl ..................................................1.0mg

Calcium pantothenate ......................................1.0mg

Riboflavin ........................................................1.0mg

p-Aminobenzoic acid.......................................0.2mg

Folic acid........................................................0.02mg

Biotin ............................................................... 2.0μg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available, without cystine,

lysine or methionine, as a premixed powder from BD

Diagnostic Systems.

Preparation of Medium: Add components to

1.0L of distilled water, omitting the specific amino

acid to be assayed for in the procedure. Heat to boil

ing for 2–3 min. Distribute into tubes. Autoclave for

10 min at 15 psi pressure–121°C.

Use: For the microbiological assay for amino acids.

Pediococcus acidilactici ATCC 8042 and Enterococ

cus hirae ATCC 8043 are used as test microorgan

isms.

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Amino Acid Assay Medium