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Anaerobe Agar (BAM M11)

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Anaerobe Agar

(BAM M11)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.5mL:

Agar ..................................................................15.0g

Pancreatic digest of casein................................15.0g

Pancreatic digest of soybean meal......................5.0g

NaCl....................................................................5.0g

L-Cysteine·HCl·H2O solution.......................... 5.0mL

Vitamin K1 solution..................................... 1.0mL

Hemin solution................................................ 0.5mL

pH 7.0 ± 0.2 at 25°C

L-Cysteine·HCl·H2O Solution:

Composition per 5.0mL:

L-Cysteine·HCl·H2O............................................0.4g

NaOH (1N solution)........................................ 5.0mL

Preparation of L-Cysteine·HCl·H2O Solu

tion: Add L-cysteine·HCl·H2O to 5.0mL 1N NaOH.

Mix thoroughly. Filter sterilize.

Hemin Solution:

Composition per 100.0mL:

Hemin .................................................................1.0g

Preparation of Hemin Solution: Add hemin to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool. Refrigerate at 4°C for

storage.

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 ..........................................................1.0g

Ethanol, absolute........................................... 20.0mL

Preparation of Vitamin K1 Solution: Add vita

min K1 to 100.0mL of 95% ethanol. Mix thoroughly.

Solution may require 2–3 days with intermittent

shaking to completely dissolve. Filter sterilize. Re

frigerate at 4°C for storage.

Preparation of Medium: Add components, ex

hemin solution and vitamin K1 solution, to distilled/

deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Adjust pH

to 7.0 ± 0.2 at 25°C. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

0.5mL of sterile hemin solution and 1.0mL of sterile

vitamin K1 solution. Mix thoroughly. Pour into ster

ile Petri dishes or distribute into sterile tubes. Reduce

medium for 24 hr by incubation in an anaerobic glove

box of GasPak jar prior to use.

Use: For the cultivation of anaerobic bacteria, such

as Brucella and Clostridium spp.

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Anaerobe Agar (BAM M11)