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Anaerobic CNA Agar (Anaerobic Colistin Nalidixic Acid Agar)

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

Anaerobic CNA Agar

(Anaerobic Colistin Nalidixic Acid Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.0g

Pancreatic digest of casein................................12.0g

NaCl....................................................................5.0g

Peptic digest of animal tissue .............................5.0g

Yeast extract........................................................3.0g

Beef extract.........................................................3.0g

Cornstarch...........................................................1.0g

Glucose ...............................................................1.0g

L-Cysteine·HCl·H2O............................................0.5g

Vitamin K1 .....................................................10.0mg

Hemin ............................................................10.0mg

Colistin...........................................................10.0mg

Nalidixic acid.................................................10.0mg

Sheep blood, defibrinated ............................. 50.0mL

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept sheep blood, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL of sterile, defibrinated sheep blood. Mix

thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of anaerobic strepto

cocci.