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Anaerobic Egg Yolk Agar

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1080.0mL:

Agar ..................................................................20.0g

Proteose peptone...............................................20.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

Egg yolk emulsion, 50%............................... 80.0mL

pH 7.0 ± 0.2 at 25°C

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

NaCl (0.9% solution).................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack 11 eggs and sepa

rate yolks from whites. Mix egg yolks with 1 chicken

egg. Measure 50.0mL of egg yolk emulsion and add

to 50.0mL of 0.9% NaCl solution. Mix thoroughly.

Filter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, 50%, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 80.0mL of sterile egg yolk emulsion,

50%. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes. Allow plates to dry at

35°C for 24 hr.

Use: For the cultivation of Clostridium species.