培养基配方

您现在的位置：网站首页 >> 培养基配方

Anaerobic Egg Yolk Agar (BAM M12)

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

Anaerobic Egg Yolk Agar

(BAM M12)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Proteose peptone...............................................20.0g

Pancreatic digest of casein..................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Egg yolk emulsion, 50%............................... 80.0mL

pH 7.0 ± 0.2 at 25°C

Egg Yolk Emulsion, 50%:

Composition per 80.0mL:

Chicken egg yolks...................................... 2 or more

NaCl (0.85% solution) .................................. 40.0mL

Preparation of Egg Yolk Emulsion, 50%:

Wash fresh eggs wtih stiff brush and drain. Soak eggs

in 70% ethanol for 1 hr. Crack eggs aseptically and

separate yolks from whites. Drain contents of yolk

sacs into sterile stoppered graduate cylinder and dis

card sacs. Measure 40.0mL of egg yolk emulsion and

add 40.0mL of 0.85% NaCl solution. Mix thoroughly

by inverting graduate cylinder. Warm to 45°–50°C.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 80.0mL sterile egg yolk emulsion. Mix thor

oughly. Pour into sterile Petri dishes. Allow plates to

dry at ambient temperature for 2–3 days or at 35°C

for 24 hr. Check plates for contamination before use.

Use: For the cultivation of Yersinia enterocolitica.