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Anaerobic TVLS Medium

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................17.0g

Beef extract.........................................................7.5g

Glucose ...............................................................6.0g

Enzymatic hydrolysate of soybean meal ............3.0g

Liver hydrolysate................................................3.0g

NaCl....................................................................2.5g

Na2SO3................................................................0.7g

Sodium thioglycolate..........................................0.5g

L-Cysteine·HCl·H2O..........................................0.25g

Agar ....................................................................0.1g

Bovine serum.............................................. 100.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept bovine serum, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 100.0mL of bovine serum. Distribute into sterile

tubes.

Use: For the isolation and cultivation of anaerobic

microorganisms.