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Andrade’s Carbohydrate Broth and Indicator (BAM M13)

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

Andrade’s Carbohydrate Broth

and Indicator

(BAM M13)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................10.0g

NaCl..................................................................10.0g

Beef extract.........................................................3.0g

Carbohydrate solution................................. 100.0mL

Andrade’s indicator....................................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems, in tubes contain

ing adonitol, arabinose, cellobiose, glucose, dulcitol,

fructose, galactose, inositol, lactose, maltose, manni

tol, raffinose, rhamnose, salicin, sorbitol, sucrose,

trehalose, or xylose.

Andrade’s Indicator

Composition per 26.0mL:

NaOH (1N solution)...................................... 16.0mL

Acid Fuchsin.....................................................0.21g

Preparation of Andrade’s Indicator: Add

Acid Fuchsin to NaOH solution and bring volume to

26.0mL with distilled/deionized water.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate..............................................5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. For glucose, lactose, sucrose,

and mannitol, add 10.0g to distilled/deionized water

and bring volume to 100.0mL. For dulcitol, salicin,

and other carbohydrates, add 5.0g to distilled/deion

ized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except

carbohydrate solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Cool. Aseptically add 100mL of sterile

carbohydrate solution to 900mL of sterile medium. Mix

thoroughly. Aseptically distribute into tubes or flasks.

Alternately, prior to autoclaving, distribute 9.0mL vol

umes into test tubes containing inverted Durham tubes.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to

25°C. Add 1.0mL of sterile carbohydrate solution to

each tube.

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Use: For the determination of carbohydrate fermenta

tion reactions of microorganisms, particularly mem

bers of the Enterobacteriaceae. A Durham tube is used

to collect gas produced during the fermentation reac

tion. Acid production is indicated by a pink color.

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Andrade’s Carbohydrate Broth and Indicator (BAM M13)