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Anthranilic Acid Medium, Revised

[所属分类:培养基配方] [发布时间:2021-11-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1040.0mL:

Na2HPO4.............................................................6.0g

KH2PO4...............................................................3.0g

NH4Cl .................................................................1.0g

NaCl....................................................................0.5g

Glucose solution ........................................... 25.0mL

CaCl2 solution............................................... 10.0mL

MgSO4 solution ............................................ 10.0mL

Anthranilic acid solution................................. 5.0mL

Glucose Solution:

Composition per 100.0mL:

D-Glucose..........................................................20.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

CaCl2 Solution:

Composition per 100.0mL:

CaCl2·2H2O ....................................................0.147g

Preparation of CaCl2 Solution: Add CaCl2 to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

MgSO4 Solution:

Composition per 10.0mL:

MgSO4·7H2O....................................................2.47g

Preparation of MgSO4 Solution: Add MgSO4

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Anthranilic Acid Solution:

Composition per 100.0mL:

Anthranilic acid ..................................................1.0g

Ethanol (95% solution)............................... 100.0mL

Preparation of Anthranilic Acid Solution:

Add anthranilic acid to 100.0mL of ethanol. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept glucose solution, CaCl2 solution, MgSO4 solu

tion, and anthranilic acid solution, to distilled/

deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 25.0mL of sterile glucose

solution, 10.0mL of sterile CaCl2 solution, 10.0mL

of sterile MgSO4 solution, and 5.0mL of sterile an-

thranilic acid solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Escherichia coli.

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Anthranilic Acid Medium, Revised