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Apple Juice Yeast Extract Medium (AJYE Medium)

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

Apple Juice Yeast Extract Medium

(AJYE Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1200.0mL:

Agar ..................................................................30.0g

Yeast extract......................................................10.0g

Apple juice......................................................... 1.0L

pH 4.8 ± 0.2 at 25°C

Preparation of Medium: Add yeast extract to

1.0L of apple juice. Mix thoroughly. Adjust pH to

4.8. Autoclave for 10 min at 9 psi pressure–114°C.

Cool to 45°–50°C. In a separate flask, add agar to

200.0mL of distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically combine the

sterile apple juice solution with the sterile agar solu

tion. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of Zymomonas species.