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APT Agar

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

APT Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................12.5g

Glucose .............................................................10.0g

Yeast extract........................................................7.5g

NaCl....................................................................5.0g

K2HPO4...............................................................5.0g

Sodium citrate.....................................................5.0g

Na2CO3 .............................................................1.25g

MnCl2·4H2O .....................................................0.14g

MgSO4·7H2O ......................................................0.8g

Polysorbate 80.....................................................0.2g

FeSO4·7H2O......................................................0.04g

Thiamine·HCl ..................................................1.0mg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 13 psi—118°–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and enumeration of bacteria,

especially heterofermentative lactobacilli, from meat

and other foods. For the cultivation of streptococci.