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Azide Blood Agar with Crystal Violet (Packer’s Agar)

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

Azide Blood Agar with Crystal Violet

(Packer’s Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

NaN3 ...................................................................0.9g

Crystal Violet ...................................................2.0mg

Sheep blood, defibrinated ............................. 50.0mL

pH 7.2 ± 0.2 at 25°C

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components, ex

cept sheep blood, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL of sterile defibrinated sheep blood. Pour

into sterile Petri dishes or distribute into sterile tubes.

Allow tubes to cool in a slanted position.

Use: For the isolation and enumeration of fecal strep

tococci from nonclinical specimens such as water and

food. Also used for the isolation of Streptococcus

pneumoniae and Erysipelothrix rhusiopathiae.