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Azide Broth (Azide Glucose Broth) (Azide Dextrose Broth)

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]
Azide Broth
(Azide Glucose Broth)
(Azide Dextrose Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g
Glucose ...............................................................7.5g
Beef extract.........................................................4.5g
NaCl....................................................................7.5g
NaN3 ...................................................................0.2g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Prepare double-strength
broth for samples larger than 1.0mL.
Use: Used in the multiple-tube technique as a pre
sumptive test for the presence of fecal streptococci.
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