培养基配方

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Bacillus fastidiosus Medium

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4·12H2O.................................................6.0g

Yeast extract........................................................2.5g

Uric acid..............................................................1.0g

Mineral solution.......................................... 100.0mL

pH 7.0 ± 0.2 at 25°C

Mineral Solution:

Composition per 100.0mL:

KH2PO4...............................................................0.1g

MgSO4·7H2O....................................................0.03g

CaCl2.................................................................0.01g

NaCl..................................................................0.01g

FeCl3·6H2O......................................................1.0mg

Preparation of Mineral Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation of Bacillus fastidiosus.