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Bacillus Medium

[所属分类:培养基配方] [发布时间:2021-11-2] [发布人:网站管理员2] [阅读次数:] [返回]

Bacillus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2HPO4........................................................1.0g

MgSO4·7H2O......................................................0.2g

KCl......................................................................0.2g

Yeast extract........................................................0.2g

Glucose solution ........................................... 50.0mL

Bromcresol Purple solution .......................... 15.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................10.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept glucose solution, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute 9.5mL volumes

into test tubes that contain an inverted Durham tube.

Autoclave for 20 min at 15 psi pressure–121°C. Cool

to 25°C. Aseptically add 0.5mL of sterile glucose to

each tube. Mix thoroughly.

Use: For cultivation and differentiation of Bacillus

species based on acid and gas production from glu

cose.