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Baird-Parker Agar

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Baird-Parker Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................17.0g

Glycine..............................................................12.0g

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

LiCl .....................................................................5.0g

Yeast extract........................................................1.0g

Sulfamethazine solution................................ 10.0mL

pH 7.0 ± 0.2 at 25°C

Sulfamethazine Solution:

Composition per 10.0mL:

Sulfamethazine..................................................0.05g

Preparation of Sulfamethazine Solution: Add

sulfamethazine to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

sulfamethazine solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

sterile sulfamethazine solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: Used as a base for the preparation of egg-tellu

rite-glycine-pyruvate agar for the selective isolation

and enumeration of coagulase-positive staphylococci

from foods.