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Baird-Parker Agar, Supplemented

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................17.0g

Glycine..............................................................12.0g

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

LiCl.....................................................................5.0g

Yeast extract........................................................1.0g

RPF supplement.......................................... 100.0mL

pH 7.0 ± 0.2 at 25°C

RPF Supplement:

Composition per 100.0mL:

Bovine fibrinogen .............................................3.75g

Trypsin inhibitor ............................................25.0mg

K2TeO3...........................................................25.0mg

Rabbit plasma ............................................... 25.0mL

Caution: Potassium tellurite is toxic.

Preparation of RPF Supplement: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept RPF supplement, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 100.0mL of filter-sterilized RPF supple

ment. Mix thoroughly but gently. Pour into sterile

Petri dishes.

Use: For the selective isolation and enumeration of

coagulase-positive staphylococci from foods.