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Balamuth Medium

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Balamuth Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 200.0mL:

Dehydrated egg yolk.........................................36.0g

Dried liver concentrate .......................................1.0g

Rice starch ..........................................................0.2g

Potassium phosphate buffer, pH 7.5 ........... 125.0mL

NaCl solution.............................................. 125.0mL

pH 7.3 ± 0.2 at 25°C

NaCl Solution

Composition per 200.0mL:

NaCl....................................................................1.6g

Preparation of NaCl Solution: Add NaCl to dis

tilled/deionized water and bring volume to 200.0mL.

Mix thoroughly.

Potassium Phosphate Buffer, 0.067M

Composition per 200.0mL:

K2HPO4 (1M solution).................................... 8.6mL

KH2PO4 (1M solution).................................. 4.66mL

Preparation of Potassium Phosphate Buffer:

Combine the K2HPO4 and KH2PO4 solutions. Bring

volume to 200.0mL with distilled/deionized water.

Adjust pH to 7.5.

Preparation of Medium: Add dehydrated egg

yolk to 36.0mL of distilled/deionized water. Add

125.0mL of 0.8% NaCl. Mix thoroughly in a blender.

Heat in a covered, double boiler until infusion reach

es 80°C and maintain at this temperature for 20 min.

Add 20.0mL of distilled/deionized H2O. Filter

through a layer of cheesecloth. To 90–100.0mL of

filtrate add 0.8% NaCl solution to bring volume to

125.0mL. Autoclave for 20 min at 15 psi pressure–

121°C. Cool to 4°C. Filter. To filtrate, add an equal

volume of 0.067M potassium phosphate buffer, pH

7.5. Add 1.0g of dried liver concentrate. Mix thor

oughly. Distribute into tubes or flasks in 10.0mL vol

umes. Autoclave for 20 min at 15 psi pressure–121°C.

Prior to inoculation, add 0.01g of rice starch to each

tube.

Use: For the cultivation and maintenance of Entam

oeba histolytica and other intestinal protozoa.